Dna Testing To Check Your Buds Background

[opietaylor]

I think it would be great if one could get their buds checked to see what strains are really in it. Today with all the over breeding no one really knows what they have. 99% of us just take a friend's or company's word on it. Does anyone know of a company that does this testing ? It would be great to know what ratio of different strains my bud has then I could better prescibe the strains to my patients.

I know with human DNA they can tell what percentage of each race a person is so it seems logical they should be able to do this with Marijuana.

 

Stop the over breeding of pot. It's diluting the gene pool. We have very little of the great strains of the 60's left. I can see 20 years from now we'll have White kush berry norhtern skunk OG diesel haze as a strain.

[rhinoman]

Hey Opie,Most of the great strains of the 60s are still here its just that nobody is willing to grow them for the fact they take so long to flower. as far as (diluting the gene pool) the gene pool has never been better and keeps getin better. i may sugest you get some good seeds from a breeder that dosent use back crossing to make there seeds. back crossing is taking short cuts and it shows in the end.

[Petyr]

You can get DNA testing done for your dog for $60. Unfortunately it isn't all that accurate because they haven't sequenced the genomes of all the 100s of different breeds of dogs.

 

Last I heard only one cannabis strain has had its genome sequenced, so we have quite a ways to go on that front. 10, maybe 20 years from now there might be something.

[Cannalytics]

Fingerprinting to prove material from two difference sources is identical is much easier than what you're proposing since it would require comparison of sequences to a database that unfortunately does not exist. As Petyr said, only one stain has been fully sequenced by http://www.medicinalgenomics.com/

[Darkmatter]

Only 2 Cannabis strains have had their Genomes mapped to date. LA confidential, and Chemdog. It was done by Medicinal Genomics in the netherlands.

[zaphod]

Last I heard only one cannabis strain has had its genome sequenced, so we have quite a ways to go on that front. 10, maybe 20 years from now there might be something.

 

The draft genome and transcriptome of Cannabis sativa

 

>>Abstract

 

Background

 

Cannabis sativa has been cultivated throughout human history as a source of fiber, oil and food, and for its medicinal and intoxicating properties. Selective breeding has produced cannabis plants for specific uses, including high-potency marijuana strains and hemp cultivars for fiber and seed production. The molecular biology underlying cannabinoid biosynthesis and other traits of interest is largely unexplored.

 

Results

 

We sequenced genomic DNA and RNA from the marijuana strain Purple Kush using shortread approaches. We report a draft haploid genome sequence of 534 Mb and a transcriptome of 30,000 genes. Comparison of the transcriptome of Purple Kush with that of the hemp cultivar 'Finola' revealed that many genes encoding proteins involved in cannabinoid and precursor pathways are more highly expressed in Purple Kush than in 'Finola'. The exclusive occurrence of Δ9-tetrahydrocannabinolic acid synthase in the Purple Kush transcriptome, and its replacement by cannabidiolic acid synthase in 'Finola', may explain why the psychoactive cannabinoid Δ9-tetrahydrocannabinol (THC) is produced in marijuana but not in hemp. Resequencing the hemp cultivars 'Finola' and 'USO-31' showed little difference in gene copy numbers of cannabinoid pathway enzymes. However, single nucleotide variant analysis uncovered a relatively high level of variation among four cannabis types, and supported a separation of marijuana and hemp.

 

Conclusions

 

The availability of the Cannabis sativa genome enables the study of a multifunctional plant that occupies a unique role in human culture. Its availability will aid the development of therapeutic marijuana strains with tailored cannabinoid profiles and provide a basis for the breeding of hemp with improved agronomic characteristics.

 

Keywords:

Cannabaceae; cannabis; marijuana; hemp; genome; transcriptome; cannabinoid<<

 

Some real good stuff in there. And it's free

 

http://genomebiology.com/2011/12/10/R102

[mrd]

Hey Opie,Most of the great strains of the 60s are still here its just that nobody is willing to grow them for the fact they take so long to flower. as far as (diluting the gene pool) the gene pool has never been better and keeps getin better. i may sugest you get some good seeds from a breeder that dosent use back crossing to make there seeds. back crossing is taking short cuts and it shows in the end.

 

Perhaps your idea of back crossing is not the conventional one. Proper back crossing is how one preserves a strain. It takes many generations to do it right. People seem to be making a lot of crosses and calling it a strain when really they are f2 generation plants which means they are 50/50 and plants grown from them will exhibit a wide variety of phenotypes as opposed to properly back crossed plants that tend to be uniform.

[MR GREENTHUMB]

thankyou very much mrd I was about to blow a seal on the backcrossing.that'show I've always heard to do it.

[rhinoman]

Perhaps your idea of back crossing is not the conventional one. Proper back crossing is how one preserves a strain. It takes many generations to do it right. People seem to be making a lot of crosses and calling it a strain when really they are f2 generation plants which means they are 50/50 and plants grown from them will exhibit a wide variety of phenotypes as opposed to properly back crossed plants that tend to be uniform.

Hey mrd, i'm fully aware what back-crossing is.and it is a good tool to determine yer f1 and p1 genotypes and thats it.

[opietaylor]

Hey Opie,Most of the great strains of the 60s are still here its just that nobody is willing to grow them for the fact they take so long to flower. as far as (diluting the gene pool) the gene pool has never been better and keeps getin better. i may sugest you get some good seeds from a breeder that dosent use back crossing to make there seeds. back crossing is taking short cuts and it shows in the end.

 

I beg to differ Rhinman, I have been invovled in the "business" for almost 45 years. You are correct that flowering, hardiness, size etc have been improved by breeding. but like any equation what you gain on one end you lose at the other. We gave up strain purity and quality for ease of growth and quanity. Before around 1975 most sinsemilla was grown from sativas. Then indica was introduced to sativa and it solved many of sativas growing problems but at a cost. By the mid 80's most sinsemilla had indica's in their gene pool. Now there are very few pure sativas grown in the N.America or Europe. The reason it is nearly impossible to get pure seeds from Thailand, Cambodia, S. America etc is that there are very few locations where indigenous farmers maintain traditional high-potency landraces.

Most all the dutch vareties contain germ plasm from a few building blocks Haze, Kush afghani or skunk. 80 % of all dutch vareties contain this germ plasm and all they do is reshuffle the deck each year. i have heard that there are a few, a very few, seed companies in amsterdam that have orginal seeds from the pre-indica breeding period. but they are held like the holy grail for to give them out would be like coke givng out their recipe.

I've been to Amsterdam a dozen times and was never greatly impressed with their weed. I'm not saying it's bad by any means. You can easily walk out of a coffeeshop like rubber man. But I never found any one hit wonders. When in amsterdam stick to the top quality hashs.

Sorry for being long winded, got carried away

[rhinoman]

hey opie, no prob with the long wind very informative. just think cannabis is dioecious so even landrace strains change over time.