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Magnolol Suppresses Metastasis In Human Prostate Carcinoma Cells

in vivo

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Magnolol, a hydroxylated biphenyl compound isolated from the root and stem bark of Magnolia officinalis, has been reported to have anticancer activity, but little is known about its molecular mechanisms of action. Increased expression of cyclooxygenase-2 (COX-2), a key enzyme in arachidonic acid metabolism, has been identified in many cancer types. Matrix metalloproteinases (MMPs) are enzymes involved in various steps of metastasis development. The objective of this study was to study the effects of magnolol on cancer invasion and metastasis using PC-3 human prostate carcinoma cells. Cellular proliferation was determined by MTT colorimetric assay. Magnolol inhibited cell growth in a dose-dependent manner. In an invasion assay conducted in Transwell chambers, magnolol showed 33 and 98% inhibition of cancer cell at 10 μM and 20 μM concentrations, respectively, compared to the control. The expression of MMP-2/-9 and COX-1/-2 was assessed by gelatin zymography and Western blot respectively. The protein and mRNA levels of both MMP-2 and MMP-9 were down-regulated by magnolol treatment in a dose-dependent manner. These results demonstrate the antimetastatic properties of magnolol in inhibiting the adhesion, invasion, and migration of PC-3 human prostate cancer cells.





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Thanks. If magnolol or magnolia officinalis sound familiar, it might be due to the previous thread on them:




Honokiol and magnolol (both found in m. officinalis) are cannabinoids that have been identified outside of cannabis. They're PPAR agonists, CB1 and CB2 partial agonists, COX-2 inhibitors, as well as displaying a host of other therapeutic properties similar to agonists found in cannabis. The difference being you can purchase 30 grams of m. officinalis extract that claims to be 95%+ magnolol/honokiol for like $15 online. I just wish I could find a lab to independently verify that claim. All in good time.

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