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Cannabinoid concentrations in hair from documented cannabis users


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Summary of this paper

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Introduction

To our knowledge, there have been no studies of deposition of cannabinoids in human hair following controlled administration of cannabis or THC. Most studies rely on self-reports of cannabis use. In cases where individuals have reasons to hide their drug use, self-report can be unreliable [21]. Go To Passage 

Subjects

They provided written informed consent to take part in controlled oral and smoked drug administration studies evaluating the pharmacokinetics and pharmacodynamics of cannabis. Subjects completed a questionnaire regarding drug use habits and had hair collected before and after drug administration. The National Institute on Drug Abuse Institutional Review Board approved the randomized, double blind, double dummy, placebo-controlled clinical studies and subjects were compensated for participation. Go To Passage 

Clinical Research Protocol

The two highest doses were in the last month of administration. The total THC administered was 116 mg. Hair specimens were collected at the end of the 10-week period 7-10 days after the last dose was administered. Go To Passage 

Hair Collection Protocol

For the purposes of this investigation and in accordance with routine hair testing practices, the first 3.9 cm of hair closest to the scalp was analyzed to reveal drug use within the last three months. A total of 53 hair specimens were collected. Hair specimens were randomized and blinded prior to analysis at American Medical Laboratories (currently Quest Diagnostics), Las Vegas, NV. Go To Passage 

Gcmsms Analysis For Thc And Thccooh In Hair

Calibration samples contained 5 and 0.5 pg/mg THC and THCCOOH, respectively. Controls in certified negative hair matrix were prepared at 0, 3.0 and 7.0 pg THC/mg hair and 0, 0.3 and 0.7 pg THCCOOH/mg hair. A blind quality control sample at a concentration within the linear range was also included with each analytical batch. Go To Passage 

Immunoassay For Cannabinoids In Hair

It was adapted and validated for the analysis of cannabinoids in human hair. The LOD of the assay was 2 pg THC equivalents/mg and a cutoff concentration of 5 pg THC equivalents/mg hair was used to screen all specimens. Intra-and interassay precisions at the cutoff concentration were 1.7 % and 9.3 %, respectively. Go To Passage 

Statistical Tests

Several procedures for detecting use of cannabis or THC were evaluated. We determined the fraction of specimens that were positive using each of the following criteria: GCMSMS for THC ≥ LOQ, GCMSMS for THCCOOH ≥ LOQ, immunoassay cannabinoids ≥ 5 pg THC equivalents/mg hair, and both immunoassay and THCCOOH ≥ respective cutoff concentrations. Fisher’s exact test, two-tailed, was used to compare the fractions of positive specimens, i.e. detection rates, for daily and non-daily users, AA and C subjects, and to test for independence of detection rates for subjects before and after smoked cannabis [22]. Go To Passage 

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Results

For those specimens with detectable cannabinoids, the range of concentrations for THC was 3.4 to > 100 pg/mg of hair and for THCCOOH 0.10 (the LOQ) to 7.3 pg/mg hair (Table 1). THC and THCCOOH concentrations were positively correlated (r = 0.38, p < 0.01, Pearson’s product moment correlation, excluding the subject with > 100 pg THC/mg hair). Median THC and THCCOOH concentrations were higher for daily users and AA subjects, but elevations were not statistically significant (Mann-Whitney Rank Sum Test, all p > 0.2, see Figures 1 and 2). Go To Passage 

Discussion

For many drugs the parent compound is in much higher concentration in hair than water-soluble metabolites and this is also true for cannabinoids. Concentrations ranged from 3.4 to > 100 pg THC/mg hair compared to 0.10 to 7.3 pg THCCOOH/mg hair. THC concentrations compared well with those reported by other investigators [7,8,10], while THCCOOH concentrations were similar to those reported by Moore et al .Go To Passage 

Conclusions

THC and THCCOOH concentrations were positively correlated (r = 0.38, p < 0.01, Pearson’s product moment correlation). Using an immunoassay cutoff concentration of 5 pg THC equivalents/mg hair, 83% of specimens that screened positive were confirmed by GCMSMS at a cutoff concentration of 0.1 pg THCCOOH/mg hair. Concentrations of (a) Δ 9 -tetrahydrocannbinol (THC) and (b) 11-nor-Δ 9tetrahydrocannbinol-9-carboxylic acid (THCCOOH) in hair of cannabis users. Go To Passage 

Source

The post Cannabinoid concentrations in hair from documented cannabis users appeared first on Michigan Medical Marijuana.

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  • 2 months later...

Yes, I know of all these tests to see cannabinoid concentrations. But this is taken from not only documented cannabis users but also random people, for example, me. When I went to do a hair transplant at https://maps.app.goo.gl/HorMNmR1u8hdzDCA9, he did this test for me, too, but I didn't understand why. But the good news is that THC was 3.4 to <100 pg/mg, so it is obvious that I am not a cannabis user, which would not have affected the passive hair loss.

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