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Top, Middle Or Bottom? Do You Know The Diffs Between Cuts Taken

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at each of those points?

The out-of-michigan-guys who did this experiment last year didn't, and in fact, didn't know if there were any diffs.  They only used Blue Cheese, so their findings may apply to only that strain.  The mediums used were Sunshine #4, another one was rockwool, and last, deep water culture hydro.  

They took sixty cuttings each of the 3 sections of the mother, and put 20 into each medium.

The cuts taken higher up rooted first and so on down the plant.  The cuts taken lower on the plant showed more failures to root and more stunted plants.

Also, the cuts taken higher up on the blue cheese mother yielded more dry meds.  With the lowest cut's yields set at 0, the mid section was +1.5%, and cuts from the upper section were up another +2.3 %, meaning clones cut from the upper stems of the mother yielded 3.8% more dry meds than clones created from the bottom of the blue cheese mother.

NOTE:  I'm reporting this rather than the study operators due to the numbers of plants that were involved.  An academic involved calculated the numbers of plants needed to make any findings not subject to chance.  Thus the numbers used.  Only one mother plant was involved.

Edited by pic book
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Besides having too many variables, what you have calculated would be one experiment with n=20.( n would be 60 in this case if you didn't add two other medium variables)  a sample size of n=20 carries hardly any statistically meaning.  What was your P value?   this is ONE experiment.  To get any statistically viable data, you would need to replicate the experiment in triplicate, then crunch the numbers.

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What garyfisher said.


This runs counter to prevailing wisdom that says you get better success from clones taken from lower branches due to higher concentrations of auxins in the older growth.  Could be that the lower branches in this case hard more woody stems, thus making it more difficult for them to root versus the softer, fresher growth up top.  I wouldn't draw any conclusions from this experiment.

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Tops are great for the win. The fleshy cambium layer of plant tops is always awaiting a "cut" as the first point of the plant most likely to suffer damage in the wild. It's like the stem cell of the plant, ready to replicate, sometimes with no help, as layer rooting, grafting....


Cambium layer- is layer of actively dividing cells between xylem and phloem tissues that is responsible for the secondary growth of stems and roots (secondary growth occurs after the first season and results in increase in thickness). Theoretically, the cambium is a single layer of cells, called initial cells; practically, it is difficult to distinguish the initials from their still-undifferentiated daughter cells, and several cell layers are collectively called the cambium, or cambial zone. Cambial cells divide to produce secondary xylem cells toward the central axis of the stem and secondary phloem cells toward the outside. The cambium originates from undifferentiated cells that have retained their embryonic capacity for continued growth and differentiation. A cambium may also form within callus tissues—masses of cells that grow over the injured surface of a wound, leading to healing.

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